These alterations might induce apoptosis in testicular germ cells.Īllium sativum (As) or garlic is a popular spice usually eaten both raw and cooked in various doses and its potential medical properties have been recognized for thousands of years. In summary, we showed that feeding with crude garlic inhibited Leydig steroidogenic enzyme expression and Sertoli cell markers. In contrast, AMH, RHOX5 and CDKN1B expression was decreased while GATA4 expression was increased. Evaluation of Sertoli markers showed that TUBB3 and GSTA2 expression was unchanged. The germ cell death process induced by As might be related to a decrease in testosterone production because of the reduced expression of steroidogenic enzymes (Star, Cyp11a, Hsd3b5 and Hsd17b). Moreover, expression of the IAP inhibitor DIABLO was increased at doses 10% and 15% of As. Expression of the caspase inhibitors BIRC3 and BIRC2 was increased at all doses of As while expression of XIAP and BIRC5 was unchanged. This cell death process was characterized by increased levels of active CASP3 but not CASP6. We showed that crude garlic-feeding induced apoptosis in testicular germ cells (spermatocytes and spermatids). Methodsĭuring one month of treatment, 24 male rats were fed 5%, 10% and 15% crude garlic. In this context, we clarify the mechanisms of action of crushed crude garlic on testicular markers. Because of garlic's chemical complexity, the processing methods and yield in preparations differ in efficacy and safety. Garlic or Allium sativum (As) shows therapeutic effects such as reduction of blood pressure or hypercholesterolemia but side-effects on reproductive functions remain poorly investigated.
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